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Image Search Results
Journal: Frontiers in Cellular Neuroscience
Article Title: CoA synthase plays a critical role in neurodevelopment and neurodegeneration
doi: 10.3389/fncel.2024.1458475
Figure Lengend Snippet: Molecular and biochemical characterization of GFAP-Coasy mice. (A) Relative Coasy mRNA expression at 15 days and 6 months in Ctrl (n = 4) and GFAP-Coasy (n = 4) mice brain, tested by qPCR. (B) Representative images of Western blot analysis and (C–J) densitometric quantification of (C) Coasy, (D) Dcx, (E) Gfap, (F) Ft-H, (G) Ft-L, (H) Dmt1, (I) TfR1, and (J) Aco2 at 15 days and 6 months in Ctrl and GFAP-Coasy mice brain. (K) Specific activity of mitochondrial respiratory chain complex I, expressed as nmoles/min/mg of protein. * p < 0.05, ** p < 0.01; *** p < 0.001 (two-way ANOVA).
Article Snippet: A quantity of 10 to 35 μg of total proteins were separated using SDS-PAGE and then electroblotted onto a nitrocellulose membrane, which was subsequently incubated with specific antibodies for COASY (PA5-28696, Thermofisher Scientific, Waltham, MA, United States), DCX (#4604, Cell Signaling, Danvers, MA, United States), GFAP (MAB360, Millipore, Burlington, MA, United States), Ft-L and Ft-H , GAPDH (ab181602, Abcam, Cambridge, United Kingdom), TfR1 (13–6,800, Thermofisher Scientific, Waltham, MA, United States), DMT1 (sc-166884, Santa Cruz Biotechnology, Dallas, TX, United States),
Techniques: Expressing, Western Blot, Activity Assay
Journal: Cell reports
Article Title: Intense Light-Mediated Circadian Cardioprotection via Transcriptional Reprogramming of the Endothelium
doi: 10.1016/j.celrep.2019.07.020
Figure Lengend Snippet: KEY RESOURCE TABLE
Article Snippet: The primary antibodies used were rabbit polyclonal PER2 (Novus Biologicals, NB100-125, Littleton CO, or Abcam, ab64460, Cambridge, MA), mouse monoclonal actin (Ab-1) (JLA20, Calbiochem, Diego, CA,), rabbit polyclonal IDH2 (Novus Biologicals, NBP2-22166, Littleton CO), rabbit polyclonal SUCLG1 (Novus Biologicals, NBP1089489, Littleton CO),
Techniques: Binding Assay, Virus, Variant Assay, Recombinant, Protein Extraction, Extraction, Isolation, Cell Culture, Membrane, Transfection, Enzyme-linked Immunosorbent Assay, Reporter Assay, Activity Assay, Colorimetric Assay, Transcription Factor Assay, Bicinchoninic Acid Protein Assay, Chromatin Immunoprecipitation, Qubit Protein Assay, SYBR Green Assay, LDH Cytotoxicity Assay, Microarray, Luciferase, Generated, shRNA, Sequencing, Control, Software